Skip to main content
. 2014 Oct;88(20):11658–11670. doi: 10.1128/JVI.00867-14

FIG 2.

FIG 2

EV71 infection of Flt3L-DCs from TLR9KO mice leads to increased viral replication and cytokine production. (A) Flt3L-DCs from WT and TLR9KO mice were cultured with EV71 at an MOI of 5 or 10 for 48 h. CpG ODN (10 μg/ml) was used as a positive control. The mRNA levels of EV71 VP1 were used as an indicator of viral replication. (B) The level of IFN-α was detected by cytokine ELISA (eBioscience, CA, USA). (C to G) TNF-α (C), IL-10 (D), MCP-1 (E), IL-6 (F), and IFN-γ (G) in culture supernatants were measured by Cytometric Bead Array (BD Bioscience, CA, USA) using a flow cytometer. (H) EV71 infection induced the death of pDCs in WT and TLR9KO mice. The pDCs were seeded at 5 × 105/well in 48 wells in a total volume of 500 μl and infected with EV71 at MOIs of 5, 10, 20, and 50. Cell viability was determined by trypan blue exclusion assay at 48 h. The data are expressed as means and SD of three independent experiments. *, P < 0.05; **, P < 0.01.