FIG 7.
Roles of pDCs in EV71 infection. (A) TLR gene expression in adult and neonatal pDCs. The cDNAs of adult (6-week-old) and neonatal (7-day-old) pDCs were isolated from wild-type mice using an anti-mPDCA1 microbead kit according to the procedures provided by the manufacturer (Miltenyi Biotec). Total RNA was extracted from pDCs using TRIzol reagent, and 1 μg of RNA was reversed transcribed to cDNA for detecting the transcripts of TLRs. Expression levels of TLR1 to -9 were assessed using a real-time qPCR assay. The data represent the mean real-time qPCR expression and SD from 3 different animals. (B) Seven-day-old pDC-depleted mice were infected with EV71 strain 4643. Antibody-treated 5-day-old mice (treated with anti-mouse CD317 antibody or control antibody) or untreated mice were infected with 1 × 107 PFU of EV71 at day 7. Clinical symptoms were monitored every 2 to 4 days. Each group included 3 to 9 mice, and all of the experiments were repeated with similar results.