TABLE 1.

UPL numbers and primer sequences for mRNA analysis by real-time PCR

Gene product	UPL no.	Primersa
		Orientation	Sequence
IL-1β	78	Forward	5′-TGTAATGAAAGACGGCACACC-3′
		Reverse	5′-TCTTCTTTGGGTATTGCTTGG-3′
GAPDH	9	Forward	5′-GAGCCAACGGGTCATCATCT-3′
		Reverse	5′-GAGGGGCCATCCACAGTCTT-3′
IFN-α	51	Forward	5′-GCCTTAACCCTCCTGGTAAAA-3′
		Reverse	5′-TCCTGTGGGAATCCAAAGTC-3′
TNF-α	25	Forward	5′-CTGTAGCCCACGTCGTAGC-3′
		Reverse	5′-TTGAGATCCATGCCGTTG-3′
IFN-γ	21	Forward	5′-ATCTGGAGGAACTGGCAAAA-3′
		Reverse	5′-TTCAAGACTTCAAAGAGTCTGAGG-3′
IL-6	6	Forward	5′-GCTACCAAACTGGATATAATCAGGA-3′
		Reverse	5′-CCAGGTAGCTATGGTACTCCAGAA-3′
IP-10	3	Forward	5′-GCTGCCGTCATTTTCTGC-3′
		Reverse	5′-TCTCACTGGCCCGTCATC-3′
MCP-1	62	Forward	5′-CATCCACGTGTTGGCTCA-3′
		Reverse	5′-GATCATCTTGCTGGTGAATGAGT-3′
MIP-1α	40	Forward	5′-CAAGTCTTCTCAGCGCCATA-3′
		Reverse	5′-GGAATCTTCCGGCTGTAGG-3′

^aSequences designed for the detection of the indicated gene products by real-time PCR are presented.