FIG 3.

Silencing of MTA1 suppressed EMT of CNE-2 cell lines both in vitro and in vivo. (A) Western blot assays showed increased levels of an epithelial marker (E-cadherin) and decreased levels of mesenchymal markers (snail, fibronectin, and vimentin) in CNE-2-siMTA1 cells compared to CNE-2-scramble cells. The blots are representative of two independent experiments, and quantification of independent experiments is on the right. All data represent means ± SEM. *, P < 0.05; **, P < 0.01 by Student's t test. (B) Wound-healing assays showed that the motility of MTA1-silenced CNE-2 cells was lower than that of control cells (n = 2 per condition). All data represent means ± SEM. *, P < 0.05 by Student's t test. (C) The silence of MTA1 decreased CNE-2 cell-invasive capacity. The numbers of invading cells in the siMTA1 and scramble groups are shown on the right (n = 3 per condition). All data represent means ± SEM. *, P < 0.05 by independent Student's t test. (D) The silence of MTA1 decreased CNE-2 cell metastasis in nude mouse tumor models. Left, representative metastatic nodules (green spots) in nude mice; right, the number of metastatic nodules formed in nude mice 3 weeks after cell injection (4 mice per group). The error bars indicate means ± SEM. *, P < 0.05 by independent Student's t test.