FIG 3.
Surface plasmon resonance (SPR) of various sAbs. SPR sensorgrams of representative sAbs binding to targets are shown. His-tagged pre- and postfusion forms of F-GCNt and HN37–565 were captured onto separate channels of a four-channel Ni-NTA surface, and sAbs were flowed over each surface at multiple concentrations. sAb 3D uniquely bound significantly to both conformations of F-GCNt (right). Raw data (black lines) and curve fits (red lines) are shown. Approximate KD values are indicated.