FIG 4.

Rta-induced autophagy is independent of the mTOR signaling pathway. 293T cells were cultured for 24 h, and lysates were prepared after 1 h treatment with dimethyl sulfoxide (lane 1) or rapamycin (Rap; 100 mM) (lane 2). 293T cells were transfected with control plasmid (C, lane 3) or pCMV-Rta (R, lane 4) and lysates were prepared at 48 h after transfection. Lysates from cells treated with rapamycin or transfected with plasmids were analyzed by immunoblotting using antibodies against the indicated proteins.