FIG 4.

Antigenic properties of native and heat-treated poliovirus. The reactivity of live (light lines) and inactivated (dark lines) PV samples with anti-PV2 MAbs was measured by ELISA. Serial dilutions of PV samples were added to 96-well plates covered with capture anti-PV2 polyclonal antibody. Anti-PV2 MAb 267 (A) or 1050 (B) was then added, and the reactivity assessed by measuring the chemical reaction after incubation with peroxidase-conjugated anti-mouse IgG. The graph shows the OD average values of duplicate wells at 492 nm of PV samples incubated at 20°C (solid lines), 50°C (dashed lines), or 60°C (dotted lines).