FIG 5.

Demodification of viral RNA from inactivated poliovirus. Aliquot samples containing viral RNA extracted from inactivated PV (0.2 D-Ag/sample) were incubated at room temperature or 70°C for periods ranging from 10 to 120 min. The DNA products obtained by RT-PCR were analyzed by agarose-gel electrophoresis (A) and real-time RT-PCR (B). Primers VP1-2521 and VP1-3125, giving a DNA product ∼600 nt in size, were used. The data represent results from three independent experiments. The average genome copy number values are shown. Standard errors are indicated as error bars. Lane M in panel A indicates DNA marker. Open circles in panel B indicate results at room temperature, and closed circles indicate the increase in DNA product (%) after incubation at 70°C.