TABLE 2.
Characterization of viral RNA from heat-treated PV samples
| Sample | Mean genome copy no. ± SDa |
|||
|---|---|---|---|---|
| Viral RNA from PV associated with L20B cells |
Viral RNA after treatment of PV with RNase A |
|||
| 50°C | 60°C | 50°C | 60°C | |
| Live PV | 1.4 ± 1.0 | 0.1 ± 0.1 | 70.9 ± 2.3 | 1.0 ± 0.0 |
| Inactivated PV | 67.2 ± 17.1 | 0.1 ± 0.1 | 84.7 ± 2.4 | 6.3 ± 5.5 |
a
The amount of viral RNA was quantified by real-time RT-PCR as. The numbers represent the genome copy number as a percentage relative to that obtained with an equivalent amount of untreated PV. The data represent results from two independent experiments.