FIG 11.

Summary diagram of HSV-2 ICP34.5 forms. (A) HSV-2 γ34.5 pre-mRNA. (B) N-terminal form of ICP34.5. The light-gray shading indicates translation extended along the pre-mRNA from the intron junction to the intron stop codon. (C) Full-length ICP34.5 and C1 and C2 forms. The black boxes indicate peptide sequences used to generate anti-34.5 antiserum. Residues conserved with HSV-1 ICP34.5 regions identified as PP1α binding and effector domains (dark-gray boxes) and the eIF2α binding domain (hatched box) are shown. Regions in HSV-2 ICP34.5 analogous to the HSV-1 ICP34.5 binding domains for beclin-1 (amino acids 60 to 85) and TBK1 (amino acids 63 to 102) are poorly conserved and therefore are not depicted.