FIG 4.

Generation of the ICP34.5 C-terminal forms is independent of the N-terminal form. Vero cells were infected with HSV-2 at an MOI of 5, transfected with empty vector, or transfected with plasmids expressing HSV-2 γ34.5 gDNA or cDNA with the indicated tags. The cells were lysed 16 h postinfection or 48 h posttransfection and prepared for Western blotting. The membranes were divided and probed using anti-ICP34.5 antiserum (A), anti-Myc antibody (B), anti-34.5 antiserum (C), or anti-HA antibody (D). The circles indicate background cellular Myc bands.