FIG 5.

The N-terminal form of ICP34.5 is translated in a variety of cell types but to differing extents. The indicated cells were left untreated (−), infected with HSV-2 333 at an MOI of 5, or transfected with p34.5gDNA-3′HA. At 18 h postinfection or 24 h posttransfection, replicate monolayers were collected and processed for Western blotting. Proteins were detected using anti-34.5 antiserum in Vero and 293 cells (A) or Vero cells and the indicated mouse and human cell types (B).