TABLE 3.

Summary of phenotypes with mutations in the SAM binding site in hMPV and VSV L proteins

Virus family	Mutant	Methylation	Attenuation in cell culturea	Attenuation in animal modelb	Immunogenicityc
Paramyxoviridae	rhMPV wild type	WTd	WT	Virulent	WT
	rhMPV G1696A	Defective in 2′-O	Attenuated	Highly attenuated	WT
	rhMPV G1700A	Defective in 2′-O	Attenuated	Attenuated	WT
	rhMPV D1755A	Defective in 2′-O	Attenuated	Highly attenuated	WT
Rhabdoviridae	rVSV wild type	WT	WT	Virulent	WT
	rVSV G1670A	Lacked G-N-7 but not 2′-O	Attenuated	Low virulence	WT
	rVSV G1674A	Sensitive to SAM concn	Not attenuated	Virulent	NDe
	rVSV D1735A	Diminished G-N-7 and 2′-O	Attenuated	ND	ND

^aAttenuation was judged by plaque size, infectivity, and growth curve. Data for VSV are from reference 27.

^bFor hMPV, attenuation was judged by viral replication and pathology in nasal turbinate and lung tissue in cotton rats; for VSV, attenuation was judged by clinical symptoms, body weight loss, viral replication, and pathology in lung and brain tissues in mice. Data for VSV are from reference 54.

^cImmunogenicity was judged by the antibody level and protection rate after challenge with virulent virus.

^dWT, wild-type level.

^eND, not done.