FIG 1.

MOBKL1B interacts with NS5A. Yeast two-hybrid analysis of the interaction between MOBKL1B and 10 HCV proteins was performed. MOBKL1B-AD (GAL4 activation domain) fusion or a control AD construct (empty) was coexpressed with core, E1, E2, p7, NS2, NS3, NS4A, NS4B, NS5A, or NS5B-DBD (GAL4 DNA binding domain) fusions or control DBD construct (empty) and tested for positive yeast two-hybrid interactions under selective nutritional conditions (lacking leucine, tryptophan, histidine, and adenosine). Each viral protein was derived from the Jc1 sequence.