FIG 1.

Replication of HSV gE-277 and HSV gE-348 mutants in rat neurons. Rat SCG neurons growing in 24-well dishes (∼40,000 neurons per well) were infected with wt HSV, HSV gE-null, HSV gE-277, or HSV gE-348 using 5 PFU/cell for 2 h; then, the cells were washed once with medium. At that time (2 h) or after 22 or 30 h, the cells were scraped into the culture medium, frozen, and sonicated, and then viral titers were determined using plaque assays with Vero cells. The experiment was performed in triplicate, and the mean titers are shown with standard deviations.