FIG 3.

Numbers of capsids and gB puncta in distal axons of SCG neurons. Rat SCG neurons growing in microfluidic devices were infected with wt HSV, HSV gE-256, HSV gE-277, HSV gE-348, HSV gE-277R, or HSV gE-348R using 8 PFU/cell by adding virus to the somal chambers. After 18 h, the devices were disassembled, and the axons in the axonal chambers were fixed with paraformaldehyde and then permeabilized with 0.1% Triton X-100 (A) or fixed and permeabilized with acetone (B) and simultaneously immunostained with antibodies specific for VP26, gB, and the microtubule-associated protein tau and then with secondary fluorescent antibodies. ImageJ software was used to count capsids and gB puncta in 10 distinct 10,551-μm² fields of the axonal compartments from three separate wells. The error bars represent standard deviations.