TABLE 1.
Functional consequences of amino acid substitutions in this studya
| Substitution | Effecta in: |
|||
|---|---|---|---|---|
| DF-1 cells |
Chickens |
|||
| Viral replication | Polymerase activity | Pathogenicity | Transmissibility | |
| WB → WB(L/PB1) | Up* | Up** | Up | — |
| LP → LP(W/PB1) | Down* | Down** | Down | — |
| WB → WB-PB1-C38Y | No change | Up** | Up* | — |
| LP(W/PB1) → LP(W/PB1-C38Y) | Up* | Up** | Up** | — |
| LP → LP-PB1-Y38C | — | Down** | Down* | Lost |
| LP → LP-PB1-V14A | No change | Down** | No change | Lost |
| WB(L/PB1) → WB(L/PB1-V14A) | Down* | Down** | Downb | No change |
a
Up, increased by the substitution; Down, decreased by the substitution; —, data not applicable; Lost, lost transmissibility by substitution. For details, see Results. Statistically significant differences were observed in each category (*, P < 0.05; **, P < 0.01).
b
Viral titers in some organs were significantly decreased.