FIG 4.

Distinct T cell receptor Vβ repertoire of gt1b_3-primed CD8⁺ T cells. Naive CD8⁺ T cells (donor D8) were primed in parallel with gt1b_3 (A and B) or with gt1b_AD (C and D) for 28 days and restimulated with the peptides gt1b_3, gt1b_1, and gt1b_AD in two separate experiments (I and II). (A and C) The frequency of IFN-γ⁺ CD8⁺ T cells upon peptide restimulation was determined. (B and D) TCR Vβ⁺ usage of IFN-γ⁺ CD8⁺ T cells upon peptide restimulation was analyzed with a panel of 24 antibodies covering approximately 70% of the TCR Vβ repertoire.