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NS31406–1415 variant-specific CD8+ T cell responses in 12 HLA-A*02-positive patients from a cohort of PWID. PBMCs from PWID were expanded in the presence of the epitope variant gt1a_1, gt1b_1, or gt1b_3, as indicated at the bottom of the graph. After 10 days, cultures were restimulated with all three epitope variants and analyzed by intracellular cytokine staining for IFN-γ. The percent frequencies of IFN-γ+ CD8+ T cells upon peptide restimulation are shown.
