FIG 2.

Identification of SARS-CoV-specific memory CD8 T cells. Prime-boost-immunized mice were rested for 42 to 45 days, and the percentages and numbers of S436 and S525-specific memory CD8 T cells were determined in BAL, lungs, and spleen. (A and B) The bar graphs show mean percentages (A) and numbers (B) of S436- and S525-specific IFN-γ⁺ CD8 T cells in the BAL, lungs, and spleen 42 to 45 days after rVV-minigene boosting. (C) The bar graphs show mean percentage of polyfunctional S436- and S525-specific IFN-γ⁺ CD8 T cells in the BAL, lungs, and spleen. Data are means of cytokine-positive CD8 T cells obtained by Boolean gating. (D) Scatter plots represent the percentages of S436 and S525 CD8 T cells that express the indicated marker. Data are representative of 2 or 3 independent experiments with 3 or 4 mice/group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (by unpaired two-tailed Student's t test).