FIG 1.

Binding of CNV VLPs to synthetic oligosaccharides. (A and B) CNV strain C33 VLPs were incubated with a panel of 26 neoglycoconjugates at 37°C (A) and a panel of 5 neoglycoconjugates at 4°C (B) immobilized on immunoplates to determine binding ability. Bound VLPs were detected using an anti-CNV antibody. Synthetic oligosaccharides were conjugated to either HSA or PAA (unlabeled). An asterisk indicates that the oligosaccharide was available for testing attached to both types of conjugate, and the mean OD₄₅₀ for both is presented. VLP binding to Lewis b was significantly different for each conjugate, and hence, each is shown independently. Schematic structures of the neoglyconjugates recognized by CNV VLPs are presented above the associated bars on the chart. (C) Neoglycoconjugates shown to bind CNV strain C33 were also incubated with two additional CNV strain VLPs (170 and HK) at 37°C. The error bars represent standard errors.