FIG 8.

Enzymatic treatment of canine samples reduces CNV VLP binding. Duodenal scrapings and intestinal tissue sections from an A antigen-positive and an A antigen-negative dog were treated with 1,2-α-fucosidase or a control for 1 h (scrapings) or 18 h (tissue sections) at 37°C. The abilities of CNV VLPs (strain C33) to bind to the scrapings or tissue sections were assessed using an ELISA-based assay (A) and immunohistochemistry (B), respectively. Confirmation that the 1,2-α-fucosidase removed H antigens was achieved by incubating treated tissue sections with Ulex (Anti-H). The error bars represent standard errors.