TABLE 1.
Neoglycoconjugates used to determine the carbohydrate specificities of CNV VLPsa
| Name | Conjugate | Structure |
|---|---|---|
| α-N-Acetylgalactose | PAA | GalNAcα1 |
| LacNAc | PAA | Galβ1-4GlcNAcβ1 |
| Forsmann disaccharide | PAA | GalNAcα1-3GalNAcβ1 |
| H type 1 | HSA | Fucα1-2Galβ1-3GlcNAcβ1 |
| H type 2 | Both | Fucα1-2Galβ1-4GlcNAcβ1 |
| H type 3 | PAA | Fucα1-2Galβ1-3GalNAcα1 |
| A heptasaccharide | HSA | GalNAcα1-3(Fucα1-2)Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glcβ1 |
| A trisaccharide | Both | GalNAcα1-3(Fucα1-2)Galβ1 |
| A disaccharide | PAA | GalNAcα1-3Galβ1 |
| A type 2b | PAA | GalNAcα1-3(Fucα1-2)Galβ1-4GlcNacβ1 |
| Lea (Lewis a) | PAA | Galβ1-3(Fucα1-4)GlcNAcβ1 |
| Leb (Lewis b) | Both | Fucα1-2Galβ1-3(Fucα1-4)GlcNAcβ1 |
| Lex (Lewis x) | PAA | Galβ1-4(Fucα1-3)GlcNAcβ1 |
| Ley (Lewis y) | Both | Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1 |
| 3-Sulfo Lex | PAA | HSO3-3Galβ1-4(Fucα1-3)GlcNAcβ1 |
| Sial Lex (sialyl-Lewis x) | Both | NeuAcα2-3Galβ1-4 (Fucα1-3)GlcNAcβ1 |
| Sial Lea (sialyl-Lewis a) | Both | NeuAcα2-3Galβ1-4 (Fucα1-3)GlcNAcβ1 |
| Sialyl LNF V (lacto N-fucopentose V) | HSA | Fucα1-2Galβ1-3(Neu5Acα2-6)GlcNAcβ1-3Galβ1-4Glc β1 |
| Sialyl LNnT (lacto-N-neotetraose) | HSA | NeuAcα2-3Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1 |
| 6-Sulfo sialyl Lex | PAA | NeuAcα2-3Galβ1-3(Fucα1-4)(HSO3-6)GlcNAcβ1 |
a
Twenty-six synthetic oligosaccharides, conjugated to either PAA or HSA, were used in ELISA-based assays to investigate the ability of CNV VLPs to bind carbohydrate structures. Ten of these synthetic oligosaccharides were only available conjugated to PAA, four were only available conjugated to HSA, and a further six oligosaccharide structures were available with either PAA or HSA conjugation.