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Soluble CD134 blocks HHV-6B infection. Soluble CD134 proteins were purified from the culture medium of 293T cells transfected with plasmids for the expression of these proteins, and expression was confirmed by Western blot (WB) analysis (top). These proteins were incubated with HHV-6B, which was then used to infect MT4 cells. The cells were harvested at 24 h postinfection and prepared for Western blot analysis (middle and bottom).
