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gQ1 and gQ2 of HHV-6B bind to CD134. The expression of each protein (indicated at the top of each lane) in the lysates of 293T cells transfected with an expression plasmid(s) was confirmed by Western blot (WB) analysis (left). Soluble forms of CD46 and CD134 (CD46FcHis and CD134FcHis, respectively) bound to Ni-NTA and were incubated with the cell lysates. The proteins bound to Ni-NTA were eluted and prepared for Western blot analysis (right).
