FIG 5.

The secreted HHV-6B gQ1/gQ2 complex binds to CD134. 293T cells were transfected with a plasmid(s) for the expression of BgQ1 (tagged with Fc and His) alone or together with BgQ2. Secreted BgQ1 was purified using Ni-NTA. The expression and secretion (lysate and medium, respectively) of BgQ1 and BgQ2 were confirmed by Western blot (WB) analysis (A). The purified proteins were incubated with CD134-expressing JJhan cells. Binding of the purified proteins to the cells was analyzed by flow cytometry using Alexa Fluor 488 goat anti-human IgG antibody (B).