FIG 6.

Analysis of the interaction between the complexes containing gQ1 mutants and CD134. (A) Amino acid sequence alignment of gQ1 proteins. The HHV-6A gQ1 sequence is in red, and the HHV-6B gQ1 sequence is in blue. (B, C, and D) 293T cells transfected with plasmids for expression of the proteins (lane numbers are defined at the bottom of the figure) were lysed for immunoprecipitation assay using anti-gH antibody. Expression of each protein (lysate) and the proteins precipitated from the lysates (anti-gH immunoprecipitation [IP]) were confirmed by Western blot (WB) analysis.