FIG 5.

ϕKZ requires translational but not transcriptional machinery of its host. (A) Phage titers of the culture supernatant of LUZ19 and ϕKZ in the absence (black) and presence (gray) of 400 μg/ml Rif, supplied to P. aeruginosa cell cultures 5 min before the addition of phage (MOI, 1). The graphs represent the average titers obtained from three independent experiments. (B) Results of primer extension analysis of phage transcripts from early (P₅₄), middle (P₁₅₂), and late (P₁₅₃) ϕKZ promoters, performed on total RNA extracted from cells infected (inf.) in the presence (+) or absence (−) of Rif. (C) Results of primer extension analysis of phage transcripts from selected early, middle, and late ϕKZ promoters on total RNA from ϕKZ-infected cells grown in the presence of Rif and chloramphenicol (Cm), added at different time points. A primer extension reaction was performed simultaneously for several phage promoters of different temporal classes, including two early promoters, P₅₃ and P₅₄, one middle promoter, P₅₀, and one late promoter, P₂₉.