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Weak inhibition of AN-1gp120 binding to RA-treated PBMCs in the presence of anti-α4 MAbs and MAdCAM-1. Binding of biotinylated AN-1gp120 to RA-treated human PBMCs preincubated with either Leu3a, L25, HP2/1, or MAdCAM-1 was tested by flow cytometry. Binding of biotinylated MAdCAM-1 to the same PBMCs preincubated with HP2/1 was used as a control. Black bars, the mean fluorescence intensity obtained by analysis with FlowJo software for each reagent.
