FIG 6.

Binding of SF162gp120 and MAdCAM-1 to α₄β₇ expressed on 293T cells. 293T cells were transfected with expression vectors for the cDNA of the human α₄ and β₇ integrins. The cell surface expression levels of the integrins were detected by flow cytometry 48 h later (top; blue and red for α₄ and β₇, respectively). (Middle) Binding of biotinylated SF162gp120 and MAdCAM-1 to transfected cells (red plots); (bottom) specificity of binding determined by testing the ability of HP2/1 and L25 to block the binding of biotinylated MAdCAM-1. Black curves, untreated cells; gray curves, cells incubated with avidin-PE only.