FIG 2.

The uropod localization of Gag is independent of Env. (A) Primary B cells were infected with full-length F-MLV encoding Gag-GFP (green) and Env-mCherry (red) and monitored by spinning disc confocal microscopy. The mean polarization index of Env-mCherry or Gag-GFP accumulation at uropods is shown at the upper right corner. (B) Primary B cells infected with F-MLV Gag-GFP (green) lacking envelope (ΔEnv). The mean polarization index of Gag-GFP accumulation at uropods is shown at the upper right corner. (C) Primary B cells infected with F-MLV Env-mCherry (red) (ΔGag) lacking Gag. The mean polarization index of Env-mCherry accumulation at uropods is shown at the upper right corner. Scale bars, 10 μm.