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PM binding and Gag multimerization target MLV Gag to the uropod. The upper scheme displays tested wild-type (wt) MLV Gag and mutants of MLV Gag. All mutations were made in a full-length virus construct with GFP fused to the C terminus of Gag. “LZ” represents the trimeric leucine zipper domain. Localization of indicated Gag-GFP mutants (green) within polarized primary B cells was determined as described for Fig. 1B. Scale bars, 10 μm.
