FIG 5.

Wild-type Gag and bm Gag do not localize to late endosome/MVB in primary B cells. (A) HEK293 cells were transfected with plasmids expressing Lamp1-YFP or YFP-Rab7 (yellow) as well as a plasmid encoding F-MLV Gag-CFP (cyan) and examined 24 h posttransfection by spinning disc confocal microcopy. A single plane of a Z-stack is displayed. (B) Primary B cells were transduced with a viral construct expressing F-MLV Gag-CFP (cyan) and immunostained for Lamp1 (red) or cotransduced with viral constructs expressing YFP-Rab7 (yellow). Images depict an entire Z-stack merged into a single extended-focus view. Scale bars, 10 μm.