FIG 7.

Wild-type and bm viruses localize to the surface of uropods in primary B cells. (A and B) Primary B cells were infected with wild-type (A) or bm (B) full-length F-MLV Gag-GFP viruses in the additional presence of wild-type and bm mutant viruses carrying GagPol. One day after infection, B cells were fixed and processed for TEM. The images to the right are magnified views of regions marked by dashed squares. The inset at the right bottom corners are magnified virus particles from indicated areas. Arrows indicate the positions of virions. Scale bars, 10 μm. (C) Quantification of uropod-localized and non-uropod-localized viral particles for wt Gag, bm Gag, and ΔPPPY Gag in TEM micrographs. Sixty, 27, and 28 polarized B cells with viral particles were analyzed for wt Gag, bm Gag, and ΔPPPY Gag, respectively. Error bars represent the standard errors of the means.