FIG 8.

Ectopic DNMT3L induces the formation of a repressive chromatin modifier complex in DNMT3L-expressing MEFs. (A) Western blot analysis of HDAC1, TRIM28, DNMT3A, DNMT3L, and SETDB1 in a MEF lysate input fraction used for immunoprecipitation. (B) DNMT3L induces formation of a HDAC1/DNMTA/SETDB1/TRIM28-containing complex in MEFs. MEFs were transfected with a control vector (+C) or a wild-type Dnmt3l vector (+Dnmt3l) or the indicated mutant expression construct for one passage. The same amounts of control IgG and anti-HDAC1 immunoprecipitates from MEF extracts were analyzed by Western blot assays using the antibodies indicated. (C) Western blot using antibodies indicated on material enriched following immunoprecipitation using anti-TRIM28 antibody.