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RNAi induced by dsP7-1 inhibited the assembly of tubules and the spread of SRBSDV in VCMs. At 24 h after transfection with dsGFP (A) or dsP7-1 (B), VCMs were inoculated with SRBSDV at an MOI of 0.04. At 36 hpi (panels I) or 84 hpi (panels II), VCMs were immunolabeled with P9-1–FITC (green) and P7-1–rhodamine (red) and then examined by confocal microscopy. Images are representative of those from more than 4 experiments. Bars, 20 μm.
