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(A) Schematic of expression plasmid pcDNA-MEF-UL12, carrying UL12 tagged with MEF. (B) 293T cells were transfected with pcDNA-MEF-UL12, harvested, and immunoprecipitated with anti-Myc and anti-Flag antibodies. Immunoprecipitates were separated in a denaturing gel and were silver stained. The upper and lower arrows indicate MEF-pUL12 and an immunoglobulin heavy chain, respectively. Molecular mass markers are indicated on the left.
