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. 2014 Sep;88(18):10624–10634. doi: 10.1128/JVI.01634-14

FIG 4.

FIG 4

Phosphorylation of pUL12 Y371 in cell cultures. (A) 293T cells were transfected with plasmid pcDNA-MEF-UL12 (diagramed in Fig. 2A), harvested at 48 h posttransfection, and lysed. The lysates were either mock treated (lanes 1) or treated with λ-PPase (lanes 2), analyzed on a Phos-tag(+) (left) or a Phos-tag(−) (right) SDS-PAGE gel, and immunoblotted with an anti-Myc antibody. (B) 293T cells were transfected with plasmid pcDNA-MEF-UL12 (lanes 1), pcDNA-MEF-UL12G336A/S338A (lanes 2), or pcDNA-MEF-UL12Y371F (lanes 3), harvested at 48 h posttransfection, and lysed. The lysates were analyzed on a Phos-tag(+) (left) or a Phos-tag(−) (right) SDS-PAGE gel. The gels were immunoblotted with an anti-Myc antibody. The pUL12 bands detected in the Phos-tag(+) SDS-PAGE gels are labeled a, b, and c.