FIG 8.

Effects of mutations in pUL12 on progeny virus yields. Vero (A and D), HEL (B and E), and A549 (C and F) cells were infected with either wild-type HSV-1(F), YK655 (ΔUL12), YK656 (ΔUL12-repair), YK660 (UL12Y371F), YK661 (UL12Y371F-repair), YK662 (UL12Y371E), YK665 (UL12G336A/S338A), or YK666 (UL12GA/SA-repair) at an MOI of 0.01 (A to C) or 3 (D to F). Total virus from the cell culture supernatants and infected cells was harvested at 48 h (A to C) or 24 h (D to F) postinfection and was assayed on 6-5 cells. Each data point is the mean ± standard error of results for triplicate samples and is representative of three independent experiments.