Immunohistochemical analysis of organs from ECTV-infected and mock-vaccinated (PBS) or MVA (105 PFU)-vaccinated IFNAR−/− mice. At day 7 (PBS) or at day 12 (MVA) postinfection, sections of tissues were immunostained (IHC) with polyclonal rabbit antibody raised against VACV Lister virions to detect ECTV antigen. Bars, 50 μm. Micrographs show representative areas of lung, liver, and spleen from a mock-vaccinated IFNAR−/− mouse (A, B, and C), or lung, liver, and spleen from an MVA-vaccinated mouse (D, E, and F).