FIG 7.

Protection of IFNAR^−/− mice requires CD8⁺ T cell-mediated immunity after low-dose MVA vaccination. (A) IFNAR^−/− mice and IFNAR^−/− mice depleted of CD8⁺ T cells (IFNAR^−/− CD8⁺) were vaccinated or mock vaccinated (IFNAR^−/− mice only) with 10⁵ PFU MVA. Weight loss of individual mice was monitored daily (3 to 5 per group). Error bars indicate SEMs, and the numbers of surviving/total animals are given in parentheses. ***, P < 0.001. (B) ECTV titers in lungs, livers and spleens of WT or CD8⁺ cell-depleted IFNAR^−/− mice after challenge infection. At the time of death or at the end of the experiments (day 21 p.i. for WT MVA-vaccinated mice), organs were removed and homogenized, and the amount of virus was determined by plaque assay (3 to 5 animals per group). Error bars indicate SEMs, and data are representative of at least two independent experiments. n.d., not detectable.