FIG 2.

PI3K signaling is essential for efficient transformation in a polyomavirus MT-driven prostate cancer model. (A, B) Lipid kinase assay (A) and Western blot analysis (B) of prostate tissue from mice with the indicated genotypes at 30 to 34 weeks. Shown are the results of one representative experiment (left) and quantifications from 3 independent experiments with standard errors of the means (right). For kinase assay quantifications, the PIP signal was normalized to the IP efficiency (IP signal/input signal in the Western blot). (C) Immunohistochemistry of formalin-fixed prostate tissue from mice with the indicated genotypes at 30 to 34 weeks.