FIG 5.
Different mechanisms for delayed PIN lesions in MT-driven transformation. (A) Hematoxylin-eosin staining of formalin-fixed prostate tissue from mice with the indicated genotypes at 56 weeks. (B) Immunohistochemistry of p-Akt and Ki67 in formalin-fixed prostate tissue sections from MT/Cre/p110αL/L mice at different ages. (C, D) MT-associated lipid kinase activity (left) and Western blot analysis (middle) of prostate tissue from 3 different mice with the indicated genotypes per time point. (Right) Quantifications for 6 samples per time point. w, weeks. The lipid kinase signal was normalized to the IP efficiency (IP signal/input signal in the Western blot). n.s., not statistically significant; **, P < 0.01. (E) Genotyping of total prostate tissue lysates from mice with the indicated genotypes at 50 weeks. Upper band, floxed allele; lower band, wild-type allele. Cre excision should lead to the complete disappearance of the floxed allele (deleted allele not detectable with the primers used).