FIG 3.

AL2 proteins are phosphorylated efficiently by SnRK1. GST (lanes 6 and 12) or GST-tagged AL2/C2 proteins from CaLCuV (lanes 1 and 7), ToMoV (lanes 2 and 8), TGMV (lanes 3 and 9), TYLCV (lanes 4 and 10), and BCTV (lanes 5 and 11) were incubated with equal molar amounts of GST-GRIK1, with GST-GRIK1 and His₆-SnRK1.1 (KD), or with GRIK1-activated SnRK1 with GRIK1 removed in phosphorylation assays in which the assay mixtures contained [γ-³²P]ATP. The reactions were resolved by SDS-PAGE followed by autoradiography to detect ³²P-labeled proteins (A). Total GST-tagged AL2/C2 proteins in the kinase reactions were monitored by immunoblotting with anti-GST antibodies (B).