FIG 5.

Conservation of AL2 S109 in subgroups of the New World begomoviruses. A phylogenetic tree was constructed by the neighbor-joining method based on the AL2/C2 protein sequence of one selected isolate from each geminivirus species listed in Table S3 in the supplemental material. Virus groups are indicated by different branch colors. The amino acid residue of each AL2/C2 protein at the equivalent CaLCuV AL2 S109 site is indicated in parentheses. The species with AL2 S109 are designated in red, and the three groups in which they cluster are numbered. Open circles, a valine residue at the equivalent position; closed circles, an aspartic acid or glutamic acid residue at the equivalent position; arrows, the viral species whose AL2/C2 proteins were characterized in this study.