FIG 6.
MVA infection induces production of neutrophil-attracting chemokines in vivo. Mice were i.n. infected with 1 × 107 PFU of MVA or the equivalent volume of PBS. (A) Analysis of chemokine expression in snap-frozen whole lungs by quantitative real-time RT-PCR 8 h p.i. Determination of relative expression values was performed as described in Materials and Methods. (B) The concentration of CXCL2 in BAL fluid was determined 24 h p.i. by multiplex bead-based technology. (C) The concentration of CCL5 in BAL fluid was determined by ELISA at the indicated time points. The dotted line intersecting the y axis indicates the lower limit of detection. The data are means and SEM (n = 3). *, P < 0.05.