FIG 4.

Characterization of type-specific and cross-reactive activity and domain-specific responses of antibodies from sera from mice vaccinated with the different genotypes. (A) ELISA reactivities of MAbs against E-protein EDIII mutant DENV-1 and DENV-2 VLPs; (B) domain-specific IgG responses to the EDIII and non-EDIII epitopes of sera from mice vaccinated with the different genotypes; (C) blocking of an HRP-labeled DENV-2-specific MAb (3H5-1) and cross-reactive MAbs (6B6C-1 and 1B7-5) by sera from mice vaccinated with the different DENV-2 genotypes containing antibodies to VLPs of the different DENV-2 genotypes determined by an epitope-blocking ELISA. ELISA data are the means ± SEMs of two independent repeat experiments. Means were analyzed by one-way ANOVA (P < 0.05), and Holm-Sidak's posttest significance is indicated with asterisks (*, P < 0.05; **, P < 0.01).