FIG 6.

The in vivo protective efficacy of the DENV-2 DNA vaccine is genotype dependent. (A to C) Groups of 2-day-old suckling mice obtained from pVD2-Asian 1-vaccinated ICR female mice (Nt₅₀ titer, >1:200) were infected intracranially with 2,000 LD₅₀s of DENV-2 strain 16681 (A), 300 LD₅₀s of DENV-2 strain 1222 (B), and 1,000 LD₅₀s of DENV-2 strain DakHD 10674 (C). The survival of the mice was monitored for 21 days after infection. Kaplan-Meier survival curves were analyzed by the log-rank test. P values of <0.05 were considered significant. The statistical significance of the difference between mice from vaccinated mothers and mice from naive mothers is indicated with asterisks. *, P = 0.0232; ****, P < 0.0001.