TABLE 2.
Oligonucleotides used in plasmid constructions
| Primer | Primer sequence (5′–3′)a | Amplified region |
|---|---|---|
| 102 forward | AATTATAGCGCTTTTCATCTGACCACACGCAAC | S14635 prM/E (aa 1–396) |
| 102 reverse | AATTATAGGCCTTGCCCAGCGTGCTTCCTTTCTTGAACCAGTCCAG | |
| 70 forward | AATTATAGCGCTTTCCATTTAACCACACGTAAT | BC100/98 prM/E (aa 1–396) |
| 70 reverse | AATTATAGGCCTTGCCCAGCGTACTTCCCTTCTTAAACCAATTGAG | |
| 85 forward | AATTATAGCGCTTTTCATTTGACCACACGCAAC | BC145/97 prM/E (aa 1–396) |
| 85 reverse | AATTATAGGCCTTGCCCAGCGTGCTTCCTTTTTTGAACCAGTTTAA | |
| 68 forward | AATTATAGCGCTTTTCATTTGACCACACGCAAC | DakHD 10674 prM/E (aa 1–396) |
| 68 reverse | AATTATAGGCCTTGCCCAGCGTGCTTCCTTTTTTGAACCAGTTTAA | |
| 16681 EDI-II forward | AATTAACGTCTCACAATGCGTTGCATAGGAATG | 16681 EDI-II (aa 1–294) |
| 16681 EDI-II reverse | AATTAACGTCTCAGCTGTAGCTTGTCCATTCTC | |
| 85 vector forward | AATTAACGTCTCGCAGCTCAAAGGAATGTCATAC | pVD2- Cosmopolitan excluding EDI-II |
| 85 vector reverse | AATTAACGTCTCCATTGTCATTGAAGGAGCGAC | |
| 68 EDI-II forward-a | AATTAACGTCTCACAATGCGATGTATAGGCATG | DakHD 10674 EDI-II (aa 1–294) |
| 68 EDI-II reverse-a | AATTAACGTCTCTTGAGTTGCAGCTTGTCCATC | |
| 16681 vector forward-a | AATTAACGTCTCGCTCAAAGGAATGTCATACTC | 16681/pVD2 excluding EDI-II |
| 16681 vector reverse-a | AATTAACGTCTCCATTGTCATTGAAGGAGTGAC | |
| 68 EDIII forward-b | AATTAACGTCTCAAAGGGATGTCGTACTCCATG | DakHD 10674 EDIII (aa 294–396) |
| 68 EDIII reverse-b | AATTAACGTCTCAGCGTGCTTCCTTTTTTGAAC | |
| 16681 vector forward-b | AATTAACGTCTCCACGCTGGGCAAGGCCTTTTC | 16681/pVD2 excluding EDIII |
| 16681 vector reverse-b | AATTAACGTCTCTCCTTTGAGCTGTAGCTTGTC | |
| pVD1i EDIII forward | AATCGTCTCAAAGGGATGTCATATGTGATGTGC | pVD1i EDIII (aa 294–396) |
| pVD1i EDIII reverse | AATTAACGTCTCAGCGTGCTTCCTTTCTTGAAC | |
| 16681 EDIII forward | AATCGTCTCAAAGGAATGTCATACTCTATGTGCAC | pVD2-Asian 1 EDIII (aa 294–396) |
| 16681 EDIII reverse | AATCGTCTCAGCGTGCTTCCTTTCTTAAACCAG | |
| pVD1i vector forward | AATTAACGTCTCCACGCTGGGCAAGGCCTTTTC | pVD1i excluding EDIII |
| pVD1i vector reverse | AATGAACGTCTCCCCTTTTAGAGTCAGTTTGTC |
a
Nucleotides in boldface are AfeI (AGCGCT), StuI (AGGCCT), and BsmBI (CGTCTC) enzyme restriction sites.