FIG 2.

Viral replication dynamics are similar in juvenile and adult AGMs. (a) Plasma viral load in adult (gray squares) and juvenile (red circles) AGMs at time points measured in weeks after inoculation with SIV_AGM. (b) Infection frequencies of sorted lymphocyte subsets from SIV_AGM-infected adult (left) and juvenile (right) AGMs, as determined by quantitative PCR for viral DNA. Naive and memory subsets were gated based on expression of CD28 and CD95. LOD denotes samples at or below the assay limit of detection. (c) Frequency of memory CD4⁺ T cells out of total CD4⁺ T cells (left) and absolute number of memory CD4⁺ T cells per microliter of blood (right) measured by flow cytometry and absolute number calculated from complete blood count. Memory CD4⁺ T cells were gated by CD3⁺ CD4⁺ CD95⁺ and CD28^hi or CD28^low expression. Adult animals are denoted by squares, juvenile animals by circles, SIV⁺ animals by filled symbols, and SIV⁻ animals by open symbols. Median values for each population are shown.